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Remove mouse or rat brains from a euthanized animal after the desired experiment and according to a standard laboratory protocol. Resting microglial cells are highly dynamic surveillants of brain parenchyma in vivo. NOTE: The conversion of files to 8-bit post-acquisition may distort the data collection. Microglia cell ramification is commonly defined as a complex shape having multiple processes and is often reported as the number of endpoints per cell and the length of cell processes. Instrumentation, objectives, and software vary amongst microscopes which will result in different sized photomicrographs despite similar objectives and change the detail as well as the number of cells within each frame.

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